Part:BBa_I716061:Design

pTRC-rbs-beta-rbs-alpha

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Source

p-TRC (Bgl2/Xho1, 4136/1713, Larger) I716060 -Cmr-rbs-beta-rbs-alpha (Bgl2/Xho1, 2949/970, Smaller) Ligated Product is I716061 -pTRC-rbs-beta-rbs-alpha (Select the [hopefully] bloodly looking colonies)